7TXE

Plasmodium falciparum Cyt c2 DSD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	54.440, 71.994, 78.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.800 - 2.300
R-factor	0.2362
Rwork	0.231
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7u2v
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.200	39.200	2.380
High resolution limit [Å]	2.300	8.900	2.300
Rmerge	0.693	0.064	7.996
Rmeas	0.696	0.065	8.035
Rpim	0.068	0.007	0.785
Total number of observations	1465254	25176	141195
Number of reflections	14274	295	1377
<I/σ(I)>	15.5	51.8	2.5
Completeness [%]	99.9	99.2	99.4
Redundancy	102.7	85.3	102.5
CC(1/2)	0.999	1.000	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	200 mM ammonium acetate, 100 mM Bis-Tris, 25% PEG3350