7TWK
Structure of a borosin methyltransferase from Mycena rosella with native peptide (MroMA1) in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-24 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 65 |
| Unit cell lengths | 191.833, 191.833, 93.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.790 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n0s |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.053 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.000 | 1.800 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.099 | 1.326 |
| Number of reflections | 183361 | 1837 |
| <I/σ(I)> | 21.2 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.8 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 282 | 5% PEG 3350, 200 mM ammonium acetate and 100 mM Tris-HCl buffer (pH=8.5) |
