7TWD

Structure of AAGAB C-terminal dimerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	47.539, 47.539, 191.394
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.250 - 2.110
R-factor	0.2172
Rwork	0.212
R-free	0.25980
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.444
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.205	1.580
Rmeas	0.213	1.658
Rpim	0.057	0.479
Number of reflections	9351	900
<I/σ(I)>	17.4	0.929
Completeness [%]	99.2	99.7
Redundancy	13.5	9.6
CC(1/2)	0.985	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	30% glycerol, 0.5 M ammonium phosphate