7TVY
Short form D7 protein from Culex quinquefasciatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-28 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.231, 122.809, 74.433 |
| Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
| Resolution | 33.230 - 1.980 |
| R-factor | 0.1944 |
| Rwork | 0.192 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Alphafold2 model |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
| Rmerge | 0.064 | 0.029 | 0.421 |
| Total number of observations | 202477 | ||
| Number of reflections | 41251 | 2112 | 1822 |
| <I/σ(I)> | 13 | ||
| Completeness [%] | 99.4 | 99.2 | 89.2 |
| Redundancy | 4.9 | 4.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 25% PEG 5000 MME, 0.2 M lithium sulfate, 0.1 M Tris HCl, pH 8.5 |
