7TVP
Viral AMG chitosanase V-Csn, E157Q mutant, chitotriose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.939, 47.421, 45.402 |
Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
Resolution | 37.130 - 1.300 |
R-factor | 0.1335 |
Rwork | 0.132 |
R-free | 0.16400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7tvl |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.130 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmeas | 0.090 | 0.964 |
Rpim | 0.034 | 0.380 |
Number of reflections | 55448 | 2455 |
<I/σ(I)> | 11.7 | 1.8 |
Completeness [%] | 97.8 | 87.9 |
Redundancy | 6.7 | |
CC(1/2) | 0.999 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME |