7TVO
Viral AMG chitosanase V-Csn, E157Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.584, 47.434, 45.460 |
Unit cell angles | 90.00, 97.89, 90.00 |
Refinement procedure
Resolution | 37.120 - 1.150 |
R-factor | 0.1193 |
Rwork | 0.118 |
R-free | 0.13500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7tvl |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.120 | 1.170 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmeas | 0.050 | 0.361 |
Rpim | 0.019 | 0.135 |
Number of reflections | 76188 | 3552 |
<I/σ(I)> | 22.1 | 5.1 |
Completeness [%] | 94.0 | 88.5 |
Redundancy | 7.2 | |
CC(1/2) | 1.000 | 0.964 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME |