7TVN
Viral AMG chitosanase V-Csn, D148N mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.760, 47.450, 45.431 |
Unit cell angles | 90.00, 97.96, 90.00 |
Refinement procedure
Resolution | 37.150 - 1.200 |
R-factor | 0.1263 |
Rwork | 0.125 |
R-free | 0.14890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7tvl |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.200 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmeas | 0.071 | 0.702 |
Rpim | 0.027 | 0.285 |
Number of reflections | 70261 | 3137 |
<I/σ(I)> | 16.1 | 2.6 |
Completeness [%] | 98.1 | 88.4 |
Redundancy | 6.7 | |
CC(1/2) | 0.999 | 0.970 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME |