7TVM
Viral AMG chitosanase V-Csn, apo structure, crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.295, 46.001, 73.556 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 38.110 - 1.350 |
| R-factor | 0.1444 |
| Rwork | 0.143 |
| R-free | 0.17420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tvl |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.110 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.060 | |
| Rpim | 0.023 | 0.454 |
| Number of reflections | 117982 | 5613 |
| <I/σ(I)> | 16.1 | 1.5 |
| Completeness [%] | 98.4 | |
| Redundancy | 6.8 | |
| CC(1/2) | 1.000 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME |
