7TVM

Viral AMG chitosanase V-Csn, apo structure, crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	163.295, 46.001, 73.556
Unit cell angles	90.00, 92.30, 90.00

Refinement procedure

Resolution	38.110 - 1.350
R-factor	0.1444
Rwork	0.143
R-free	0.17420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tvl
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.110	1.370
High resolution limit [Å]	1.350	1.350
Rmeas	0.060
Rpim	0.023	0.454
Number of reflections	117982	5613
<I/σ(I)>	16.1	1.5
Completeness [%]	98.4
Redundancy	6.8
CC(1/2)	1.000	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME