7TVL
Viral AMG chitosanase V-Csn, apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.72929 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.836, 47.632, 45.546 |
Unit cell angles | 90.00, 97.82, 90.00 |
Refinement procedure
Resolution | 37.180 - 0.890 |
R-factor | 0.1203 |
Rwork | 0.120 |
R-free | 0.13070 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.200 | 0.910 |
High resolution limit [Å] | 0.890 | 0.890 |
Rmeas | 0.093 | |
Rpim | 0.036 | 0.549 |
Number of reflections | 174574 | 7896 |
<I/σ(I)> | 11.4 | |
Completeness [%] | 99.1 | |
Redundancy | 6.6 | |
CC(1/2) | 0.998 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME |