7TVC
Short form D7 protein from Aedes aegypti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 |
| Unit cell lengths | 85.653, 85.653, 30.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.280 - 1.190 |
| R-factor | 0.1587 |
| Rwork | 0.158 |
| R-free | 0.17080 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.280 | 1.210 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.068 | 0.456 |
| Number of reflections | 72163 | 3265 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.2 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20% PEG 6000, 0.1M MES, pH 6 |
