7TVC
Short form D7 protein from Aedes aegypti
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 4 |
Unit cell lengths | 85.653, 85.653, 30.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.280 - 1.190 |
R-factor | 0.1587 |
Rwork | 0.158 |
R-free | 0.17080 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.280 | 1.210 |
High resolution limit [Å] | 1.190 | 1.190 |
Rmerge | 0.068 | 0.456 |
Number of reflections | 72163 | 3265 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 99.2 | |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20% PEG 6000, 0.1M MES, pH 6 |