7TVB
Stat5A Core in Complex with AK305
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.1271 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 239.937, 239.937, 113.224 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.990 - 2.653 |
| R-factor | 0.2107 |
| Rwork | 0.209 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Previously solved structure in lab |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.960 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 7.190 | 2.650 |
| Rmerge | 0.095 | 0.032 | 0.976 |
| Rmeas | 0.097 | 0.033 | 1.005 |
| Rpim | 0.021 | 0.007 | 0.237 |
| Total number of observations | 741459 | ||
| Number of reflections | 36049 | 1875 | 1795 |
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 99.9 | 100 | 99.9 |
| Redundancy | 20.6 | 21.5 | 17.2 |
| CC(1/2) | 1.000 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1M Na Malonate pH 7.0, 1% Jeffamine pH 7.0, 5% peg 400 |
