7TV2

X-ray crystal structure of HIV-2 CA protein CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-27
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	48.813, 30.919, 46.494
Unit cell angles	90.00, 90.56, 90.00

Refinement procedure

Resolution	24.410 - 1.980
R-factor	0.2056
Rwork	0.203
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6axx
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.120	26.120	2.030
High resolution limit [Å]	1.980	9.070	1.980
Rmerge	0.058	0.030	0.257
Rmeas	0.072	0.037	0.318
Rpim	0.041	0.022	0.184
Total number of observations	12687	146	908
Number of reflections	4761	54	348
<I/σ(I)>	11.1	21.5	3.1
Completeness [%]	96.3	90.1	96.4
Redundancy	2.7	2.7	2.6
CC(1/2)	0.997	0.999	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.2 M tri-sodium citrate, 0.1M Bis-Tris propane, pH 7.0