7TUV
Crystal structure of the exoribonucleolytic module of T. brucei RRP44
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.296, 91.113, 148.305 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 77.630 - 2.225 |
R-factor | 0.1977 |
Rwork | 0.194 |
R-free | 0.26470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model |
RMSD bond length | 0.009 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.632 | 2.554 |
High resolution limit [Å] | 2.225 | 2.225 |
Number of reflections | 24678 | 1235 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 93.8 | 70.6 |
Redundancy | 24 | |
CC(1/2) | 0.998 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 15% (w/v) PEG 20000, 0.1 M MES pH 6.0 |