7TUN
Crystal structure analysis of human CKB complex with a covalent compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 101.240, 101.240, 160.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.590 - 2.930 |
R-factor | 0.2074 |
Rwork | 0.205 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dre |
Data reduction software | XDS |
Data scaling software | xia2 (3.5.0) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 85.680 | 85.720 | 2.980 |
High resolution limit [Å] | 2.930 | 7.950 | 2.930 |
Rmeas | 0.299 | 0.070 | 2.618 |
Rpim | 0.083 | 0.020 | 0.704 |
Total number of observations | 242855 | 11810 | 12739 |
Number of reflections | 18694 | 1067 | 932 |
<I/σ(I)> | 9 | 28.2 | 1.1 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 13 | 11.1 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.7M Ammonium citrate, pH 7.4 |