7TUH

Crystal structure of anti-tapasin PaSta2-Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	273
Detector technology	PIXEL
Collection date	2020-02-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	49.104, 62.127, 128.462
Unit cell angles	90.00, 90.04, 90.00

Refinement procedure

Resolution	64.230 - 2.300
R-factor	0.2214
Rwork	0.218
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2uyl
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.230	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.109
Rmeas	0.119
Rpim	0.049	0.866
Number of reflections	34309	3406
<I/σ(I)>	8.66	0.78
Completeness [%]	99.0	98.81
Redundancy	5.8
CC(1/2)	0.997	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	14% PEG 6000, 0.1M Na. Cacodylate