7TUF
Crystal structure of Tapasin in complex with PaSta1-Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2020-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.422, 68.988, 142.981 |
| Unit cell angles | 90.00, 108.42, 90.00 |
Refinement procedure
| Resolution | 47.450 - 2.800 |
| R-factor | 0.2329 |
| Rwork | 0.231 |
| R-free | 0.27420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f8u |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.642 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.087 | |
| Rmeas | 0.102 | |
| Rpim | 0.052 | 0.821 |
| Number of reflections | 527753 | 5107 |
| <I/σ(I)> | 10.44 | 1.05 |
| Completeness [%] | 97.7 | 95.32 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.998 | 0.483 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG 4000, 0.1M Hepes |
