7TUF

Crystal structure of Tapasin in complex with PaSta1-Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	273
Detector technology	PIXEL
Collection date	2020-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	117.422, 68.988, 142.981
Unit cell angles	90.00, 108.42, 90.00

Refinement procedure

Resolution	47.450 - 2.800
R-factor	0.2329
Rwork	0.231
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f8u
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.087
Rmeas	0.102
Rpim	0.052	0.821
Number of reflections	527753	5107
<I/σ(I)>	10.44	1.05
Completeness [%]	97.7	95.32
Redundancy	3.8	3.7
CC(1/2)	0.998	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	10% PEG 4000, 0.1M Hepes