7TU3

Structure of the L. blandensis dGTPase del55-58 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	181.410, 181.410, 110.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.890 - 2.170
R-factor	0.1944
Rwork	0.194
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bg2
RMSD bond length	0.009
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.300
High resolution limit [Å]	2.170	2.170
Rmerge	0.154	1.243
Rmeas	0.160	1.289
Number of reflections	97009	14855
<I/σ(I)>	8.88	1.04
Completeness [%]	99.2	95.4
Redundancy	14.8	14.1
CC(1/2)	0.996	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Crystal grown in 1ul:1ul drops of well solution (0.6M Sodium Potassium Tartrate, 0.2M Lithium Sulfate, 0.1M Bis-Tris pH 6.5) and 10mg/mL protein. Cryoprotected by soaking in well solution with 30% ethylene glycol added