7TTY
Crystal structure of potent neutralizing antibody 10-40 in complex with bat WIV1 receptor-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2021-12-12 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.311, 69.546, 95.215 |
Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
Resolution | 91.690 - 3.110 |
R-factor | 0.244 |
Rwork | 0.235 |
R-free | 0.32930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7sd5 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.917 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 91.690 | 10.000 | 3.410 |
High resolution limit [Å] | 3.110 | 8.000 | 3.190 |
Rmerge | 0.190 | 0.257 | 1.790 |
Rmeas | 0.400 | 0.279 | 0.900 |
Rpim | 0.165 | 0.107 | 0.801 |
Number of reflections | 11493 | 524 | 1930 |
<I/σ(I)> | 2 | ||
Completeness [%] | 99.6 | 99.9 | 99.8 |
Redundancy | 6.8 | 6.6 | 6.9 |
CC(1/2) | 0.943 | 0.956 | 0.352 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium citrate, pH 5.5, 20% propanol, 20% PEG4000 |