7TTM
Crystal structure of potent neutralizing antibody 10-40 in complex with Sarbecovirus bat SHC014 receptor-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2021-10-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979110 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.433, 69.217, 81.853 |
Unit cell angles | 90.00, 103.63, 90.00 |
Refinement procedure
Resolution | 79.360 - 2.240 |
R-factor | 0.2041 |
Rwork | 0.201 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7sd5 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 79.500 | 2.720 | 2.320 |
High resolution limit [Å] | 2.240 | 2.600 | 2.240 |
Rmerge | 0.182 | 1.873 | 0.106 |
Rmeas | 0.218 | 2.036 | 0.115 |
Rpim | 0.083 | 0.786 | 0.043 |
Number of reflections | 37400 | 2656 | 613 |
<I/σ(I)> | 6.4 | ||
Completeness [%] | 98.6 | 91 | 99.4 |
Redundancy | 6.7 | 6.3 | 6.6 |
CC(1/2) | 0.990 | 0.315 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium citrate, pH 5.2, 28% PEG4000, 0.2 M ammonium acetate |