7TSQ

Structure of Enterobacter cloacae Cap2 bound to CdnD02 C-terminus, AMP state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	49.695, 76.354, 126.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.420 - 2.110
R-factor	0.2035
Rwork	0.202
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7to3
RMSD bond length	0.008
RMSD bond angle	0.878
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.900	2.180
High resolution limit [Å]	2.110	2.110
Rmerge	0.146	0.860
Rpim	0.062	0.384
Number of reflections	28304	2237
<I/σ(I)>	15.3	2.5
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.993	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris-HCl pH 8.5, 0.2 M MgCl2, and 30% PEG 3350