7TRW
Crystal Structure of the C-terminal Ligand-Binding Domain of the LysR family Transcriptional Regulator YfbA from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-15 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97930 |
Spacegroup name | P 62 |
Unit cell lengths | 86.073, 86.073, 69.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.270 - 2.280 |
R-factor | 0.1997 |
Rwork | 0.198 |
R-free | 0.23410 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.591 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.197 | 1.366 |
Rmeas | 0.203 | 1.540 |
Rpim | 0.048 | 0.670 |
Number of reflections | 13379 | 655 |
<I/σ(I)> | 21.9 | 0.91 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 16.4 | 4.6 |
CC(1/2) | 1.000 | 0.388 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 1.03 M Sodium/Potassium Phosphate, pH 5.0, 150 mM Malate, 3.0 % v/v PEG 300 |