EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS TALOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Phase Plate | VOLTA PHASE PLATE |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.4 |
Software | cryoSPARC,RELION,EMAN |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |