7TR3
CaKip3[2-482] - AMP-PNP in complex with a dolastatin-10-stabilized tubulin ring
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.9 |
| Software | UCSF Chimera,Coot,RELION,Leginon,RosettaEM,PHENIX,MODELLER |
| Symmetry | C14 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
