7TQB
Crystal structure of monoclonal S9.6 Fab bound to DNA-RNA hybrid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.426, 158.884, 43.142 |
Unit cell angles | 90.00, 105.15, 90.00 |
Refinement procedure
Resolution | 37.091 - 3.100 |
R-factor | 0.2317 |
Rwork | 0.229 |
R-free | 0.27190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tt1 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.500 | 3.130 |
High resolution limit [Å] | 3.020 | 3.020 |
Number of reflections | 9509 | 925 |
<I/σ(I)> | 3.78 | 1.55 |
Completeness [%] | 98.7 | |
Redundancy | 12.1 | |
CC(1/2) | 0.841 | 0.393 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 1000 0.2 M sodium narrate |