7TQA
Crystal Structure of monoclonal S9.6 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 31 |
Unit cell lengths | 96.649, 96.649, 140.583 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.700 - 2.328 |
R-factor | 0.182 |
Rwork | 0.179 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | OTHER PDB DEPOSITION IN THIS WORK |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.700 | 2.411 |
High resolution limit [Å] | 2.328 | 2.328 |
Number of reflections | 62725 | 6284 |
<I/σ(I)> | 9.24 | 1.55 |
Completeness [%] | 99.6 | 99.35 |
Redundancy | 14.6 | |
CC(1/2) | 0.996 | 0.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M ammonium sulfate, 50 mM Bis-Tris, 24% PEG 8000 |