7TNV
Crystal Structure Of Human NADH-Cytochrome B5 Reductase T117S Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-27 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.541780 |
| Spacegroup name | P 41 |
| Unit cell lengths | 65.530, 65.530, 71.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.340 - 1.930 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.615 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.340 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.070 | |
| Rmeas | 0.073 | 1.602 |
| Number of reflections | 22991 | 1640 |
| <I/σ(I)> | 21.8 | 2.55 |
| Completeness [%] | 99.8 | |
| Redundancy | 12.6 | |
| CC(1/2) | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl pH 7.8, PEG 4K 10% |
