7TN1

Multistate design to stabilize viral class I fusion proteins

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	170.468, 170.468, 171.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.280 - 3.100
R-factor	0.2563
Rwork	0.254
R-free	0.31510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c6b
RMSD bond length	0.010
RMSD bond angle	1.283
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.280	3.211
High resolution limit [Å]	3.100	3.100
Rmerge		1.336
Number of reflections	44788	4518
<I/σ(I)>	6.32	1.72
Completeness [%]	96.0	99.47
Redundancy	6.4	7.2
CC(1/2)	0.981	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Sodium formate, 20% w/v PEG 3,350