7TLW
Murine Meteorin-like C-terminal NTR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.999, 80.162, 51.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.990 - 1.750 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20630 |
| Structure solution method | SAD |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.600 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.062 | 0.552 |
| Number of reflections | 13370 | 1902 |
| <I/σ(I)> | 27.4 | 5.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Bis-Tris propane, pH 7.0, 1.5 M ammonium chloride, 25 mM ethylenediamine dihydrochloride |
