7TLH
Murine meteorin C-terminal NTR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.25 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.757, 75.672, 75.725 |
| Unit cell angles | 90.00, 128.50, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.740 |
| R-factor | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.090 | 1.210 |
| Number of reflections | 55117 | 8027 |
| <I/σ(I)> | 10.2 | 1.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Bis-Tris, pH 5.4, 1 M ammonium sulfate, 1% w/v PEG3350 |
