7TL4
Crystal Structure of Yeast p58C Multi-Tyrosine Mutant 6YF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-06 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.519, 51.488, 90.027 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.606 - 1.805 |
R-factor | 0.1971 |
Rwork | 0.196 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dtz |
RMSD bond length | 0.016 |
RMSD bond angle | 1.253 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.085 | 0.064 | 0.338 |
Rmeas | 0.095 | 0.071 | 0.381 |
Rpim | 0.041 | 0.031 | 0.172 |
Total number of observations | 90999 | ||
Number of reflections | 16970 | 1798 | 1489 |
<I/σ(I)> | 13.7 | ||
Completeness [%] | 97.0 | 94.3 | 87.5 |
Redundancy | 5.4 | 5.2 | 4.4 |
CC(1/2) | 0.990 | 0.965 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Protein drops contained 20 mM HEPES (pH 6.8), 75 mM NaCl, 2 mM DTT mixed with equal volume of 100 mM TRIS (pH 8.5) and 55-70% MPD. |