7TL4
Crystal Structure of Yeast p58C Multi-Tyrosine Mutant 6YF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-06 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.519, 51.488, 90.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.606 - 1.805 |
| R-factor | 0.1971 |
| Rwork | 0.196 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dtz |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.253 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.085 | 0.064 | 0.338 |
| Rmeas | 0.095 | 0.071 | 0.381 |
| Rpim | 0.041 | 0.031 | 0.172 |
| Total number of observations | 90999 | ||
| Number of reflections | 16970 | 1798 | 1489 |
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 97.0 | 94.3 | 87.5 |
| Redundancy | 5.4 | 5.2 | 4.4 |
| CC(1/2) | 0.990 | 0.965 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Protein drops contained 20 mM HEPES (pH 6.8), 75 mM NaCl, 2 mM DTT mixed with equal volume of 100 mM TRIS (pH 8.5) and 55-70% MPD. |
