7TKZ
Crystal structure of D94A human Galectin-7 mutant in presence of lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.230, 76.400, 111.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.930 - 1.830 |
R-factor | 0.1693 |
Rwork | 0.166 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gal |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.12) |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.940 | 44.940 | 1.900 |
High resolution limit [Å] | 1.830 | 3.940 | 1.830 |
Rmerge | 0.074 | 0.054 | 0.248 |
Rmeas | 0.081 | 0.059 | 0.271 |
Rpim | 0.107 | 0.024 | 0.107 |
Number of reflections | 23542 | 2530 | 2344 |
<I/σ(I)> | 6.8 | 28.3 | 6.8 |
Completeness [%] | 99.9 | 99.8 | 99.9 |
Redundancy | 6.2 | 6 | 6.3 |
CC(1/2) | 0.997 | 0.997 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.1 M NaCl, 0.1 M Tris pH 8.0, 20 % PEG 6000, 15% glycerol, 7.5 mM Lactose |