7TJP

HIV-1 gp120 complex with CJF-II-195

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.210, 121.635, 194.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.740 - 2.770
R-factor	0.227
Rwork	0.224
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tgr
RMSD bond length	0.012
RMSD bond angle	1.716
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.040
High resolution limit [Å]	2.770	2.770
Rmerge	0.252	2.107
Number of reflections	31647	1582
<I/σ(I)>	10.1
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.997	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14%(w/v) PEG 1500, 0.1 M calcium chloride, 0.1 M imidazole pH 6.5