7TJA
Structure of the Light Harvesting Complex PE545 from Proteomonas sulcata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.216, 132.478, 93.892 |
| Unit cell angles | 90.00, 117.35, 90.00 |
Refinement procedure
| Resolution | 29.380 - 1.960 |
| R-factor | 0.1796 |
| Rwork | 0.176 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xg0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.976 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.380 | 29.380 | 2.010 |
| High resolution limit [Å] | 1.960 | 8.760 | 1.960 |
| Rmerge | 0.109 | 0.035 | 0.877 |
| Rmeas | 0.119 | 0.038 | 0.948 |
| Rpim | 0.045 | 0.015 | 0.356 |
| Total number of observations | 939381 | 10320 | 60197 |
| Number of reflections | 135804 | 1545 | 8888 |
| <I/σ(I)> | 11.6 | 37.6 | 2 |
| Completeness [%] | 99.0 | 97.1 | 87.9 |
| Redundancy | 6.9 | 6.7 | 6.8 |
| CC(1/2) | 0.998 | 0.999 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% 3350 PEG, 50 mM HEPES, 100 mM MgCl2 |
