7THB
Crystal structure of an RNA-5'/DNA-3' strand exchange junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.025, 43.586, 52.182 |
| Unit cell angles | 92.06, 103.72, 99.95 |
Refinement procedure
| Resolution | 35.340 - 1.640 |
| R-factor | 0.2391 |
| Rwork | 0.237 |
| R-free | 0.28410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | see methods in primary citation |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.416 |
| Data reduction software | XDS |
| Data scaling software | STARANISO (3.339) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.340 | 35.335 | 1.781 |
| High resolution limit [Å] | 1.637 | 5.089 | 1.637 |
| Rmerge | 0.037 | 0.028 | 1.293 |
| Rmeas | 0.041 | 0.031 | 1.410 |
| Rpim | 0.016 | 0.013 | 0.556 |
| Number of reflections | 24808 | 1240 | 1240 |
| <I/σ(I)> | 15.641 | 44.8 | 1.5 |
| Completeness [%] | 64.7 | 97.7 | 14.5 |
| Redundancy | 6 | 5.7 | 6.3 |
| CC(1/2) | 0.999 | 0.999 | 0.474 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 0.5 uL of sample was combined with 0.5 uL reservoir solution (0.05 M sodium succinate (pH 5.3), 0.5 mM spermine, 20 mM magnesium chloride, 2.6 M ammonium sulfate) over 500 uL reservoir solution. |
