7TGN
Crystal structure of DesD, the desferrioxamine synthetase from the Streptomyces violaceus salmycin biosynthetic pathway
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 113 |
Detector technology | PIXEL |
Collection date | 2020-06-20 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 108.530, 108.530, 517.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.470 - 2.300 |
R-factor | 0.1966 |
Rwork | 0.196 |
R-free | 0.22970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.470 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.119 | |
Rpim | 0.032 | 0.519 |
Number of reflections | 138685 | 10146 |
<I/σ(I)> | 21.2 | 1.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 28 | |
CC(1/2) | 1.000 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 90-100 mM Potassium citrate 25% PEG 8000 50 mM MES |