7TGF

Single-domain VHH intrabodies neutralize ricin toxin.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	50.830, 50.830, 260.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.886 - 1.347
R-factor	0.1594
Rwork	0.158
R-free	0.18070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rtc 6cwg
RMSD bond length	0.011
RMSD bond angle	1.102
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.370
High resolution limit [Å]	1.347	3.660	1.350
Rmerge	0.074	0.044	2.084
Rmeas	0.077	0.046	2.213
Rpim	0.022	0.013	0.735
Number of reflections	77109	4307	3793
<I/σ(I)>	5.3
Completeness [%]	100.0	99.9	100
Redundancy	11.7	11.8	8.7
CC(1/2)		0.999	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	100 mM sodium citrate pH 4.0, 30% PEG 6000, and 1 M lithium chloride