7TGC
[T:Ag+/Hg2+:T--(pH8.5-pH11; 90s in metals)] Metal-mediated DNA base pair in tensegrity triangle grown at pH 8.5 and soaked in pH 11 with Ag+ and Hg2+ for 90s
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00743 |
| Spacegroup name | H 3 |
| Unit cell lengths | 106.941, 106.941, 88.902 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.570 - 5.190 |
| R-factor | 0.2487 |
| Rwork | 0.247 |
| R-free | 0.31260 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.140 | 5.860 |
| High resolution limit [Å] | 5.190 | 5.190 |
| Number of reflections | 2408 | 406 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 87.1 | |
| Redundancy | 10.5 | |
| CC(1/2) | 0.996 | 0.226 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Tris, magnesium sulfate, silver nitrate, mercury chloride |
