7TFM
Atomic Structure of the Leishmania spp. Hsp100 N-Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 60.971, 60.971, 71.807 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.963 - 1.055 |
R-factor | 0.1345 |
Rwork | 0.134 |
R-free | 0.15280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wbw |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.070 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmeas | 0.591 | |
Rpim | 0.023 | 0.259 |
Number of reflections | 61983 | 2900 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 97.1 | |
Redundancy | 9.1 | |
CC(1/2) | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 5% PEG 2000, 5% PEG 3350, 5% PEG 4000, 5% PEGME 5000, 0.1 M KCl, 0.1 M MgCl2, 0.1 M PIPES pH 7.0 |