7TEN
Crystal structure of the Listeria monocytogenes GS-Met-Sox-P- ADP complex to 3.5 Angstrom
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 112.608, 137.613, 138.023 |
| Unit cell angles | 60.75, 87.16, 68.40 |
Refinement procedure
| Resolution | 63.880 - 3.500 |
| R-factor | 0.2035 |
| Rwork | 0.201 |
| R-free | 0.27260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tdp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.880 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rpim | 0.130 | 0.966 |
| Number of reflections | 62204 | 6451 |
| <I/σ(I)> | 4.8 | 1.2 |
| Completeness [%] | 74.7 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.995 | 0.323 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15% PEG 4000, 40 mM potassium phosphate dibasic pH 7.5, 20% glycerol |
