7TEC
Structure of the Listeria monocytogenes GlnR-DNA complex to 3.45 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.09 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 28.632, 47.717, 121.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.510 - 3.450 |
R-factor | 0.2701 |
Rwork | 0.266 |
R-free | 0.30910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7tea |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.510 | 4.340 |
High resolution limit [Å] | 3.450 | 3.450 |
Rpim | 0.038 | 0.240 |
Number of reflections | 2384 | 237 |
<I/σ(I)> | 8.9 | 2 |
Completeness [%] | 96.8 | |
Redundancy | 4.1 | |
CC(1/2) | 0.998 | 0.710 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 275 | 50 mM sodium cacodylate pH 6.5, 35% MPD, 10 mM MgCl2 |