7TE4

Crystal structure of Fab2 anti-GluN2B antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	54.744, 66.901, 240.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.229 - 2.456
R-factor	0.2627
Rwork	0.261
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b3j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.499
High resolution limit [Å]	2.456	2.456
Rmerge	0.182	1.168
Number of reflections	33229	2877
<I/σ(I)>	10.3
Completeness [%]	99.9
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	20% w/w PEG3350, 8% Tacsimate