7TE3
Crystal Structure of a Double Loop Deletion Mutant in gC1qR/C1qBP/HABP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 80.529, 80.529, 114.552 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.990 - 2.200 |
| R-factor | 0.2384 |
| Rwork | 0.233 |
| R-free | 0.28780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p32 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.882 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.143 | 0.040 | 1.865 |
| Rmeas | 0.147 | 0.041 | 1.936 |
| Rpim | 0.032 | 0.009 | 0.499 |
| Total number of observations | 239844 | ||
| Number of reflections | 11799 | 1324 | 1122 |
| <I/σ(I)> | 6 | ||
| Completeness [%] | 99.9 | 99.6 | 99.6 |
| Redundancy | 20.3 | 19.4 | 13.8 |
| CC(1/2) | 1.000 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M BisTris-HCl pH 6.5, 0.1 M sodium chloride, 1.5 M ammonium sulfate |
