7TDQ

Crystal structure of KSHV KicGAS/ORF52

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-07-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	53.332, 58.683, 138.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.770 - 2.500
R-factor	0.2286
Rwork	0.225
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oa5
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.128	0.595
Rmeas	0.142	0.696
Rpim	0.062	0.351
Number of reflections	15886	1481
<I/σ(I)>	19.78	1.32
Completeness [%]	98.3	90.4
Redundancy	4.9	2.9
CC(1/2)	0.991	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	1 microL of protein (10 mg/ml) + 1 microL of reservoir solution of 9% (v/v) PEG3350, 100mM Na-citrate pH 4.5 + 0.2microL of the detergent additive n-Octyl-N,N-dimethyl-3-ammonio-1-propanesulfonate