7TCU
Methanobactin biosynthetic protein complex of MbnB and MbnC from Methylosinus trichosporium OB3b at 2.31 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-10 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.722 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.670, 215.680, 215.624 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.330 - 2.310 |
R-factor | 0.2038 |
Rwork | 0.202 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7tcr |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.330 | 49.330 | 2.370 |
High resolution limit [Å] | 2.310 | 10.350 | 2.310 |
Rmerge | 0.092 | 0.064 | 0.691 |
Rmeas | 0.108 | 0.076 | 0.817 |
Total number of observations | 341251 | ||
Number of reflections | 52288 | 1093 | 6965 |
<I/σ(I)> | 8.49 | 17.17 | 1.7 |
Completeness [%] | 99.1 | 98.1 | 95.6 |
Redundancy | 3.458 | 3.682 | 3.435 |
CC(1/2) | 0.992 | 0.990 | 0.802 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283.15 | 0.2 M lithium sulfate, 0.1 M Bis-Tris propane, pH 7.0, 20-26% w/v PEG3350 |