7TCR
Methanobactin biosynthetic protein complex of MbnB and MbnC from Methylosinus trichosporium OB3b at 2.62 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-05 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.722 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.780, 215.640, 214.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.500 - 2.620 |
R-factor | 0.2056 |
Rwork | 0.204 |
R-free | 0.23740 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.566 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 2.714 |
High resolution limit [Å] | 2.620 | 2.620 |
Rmerge | 0.124 | 0.804 |
Rmeas | 0.127 | 0.822 |
Rpim | 0.025 | 0.169 |
Number of reflections | 35237 | 3396 |
<I/σ(I)> | 26.12 | 5.47 |
Completeness [%] | 99.3 | 99.12 |
Redundancy | 23.1 | 23 |
CC(1/2) | 0.996 | 0.976 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 283.15 | 0.2 M lithium sulfate, 0.1 M HEPES, pH 7, Bis-Tris, pH 6.5, 25% w/v PEG3350 |