7TCB
Crystal Structure of the YaeQ Family Protein VPA0551 from Vibrio parahaemolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.024, 47.687, 115.519 |
Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
Resolution | 40.920 - 2.700 |
R-factor | 0.2253 |
Rwork | 0.224 |
R-free | 0.26470 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.439 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.124 | 0.799 |
Number of reflections | 24176 | 844 |
<I/σ(I)> | 15.5 | 1.3 |
Completeness [%] | 95.1 | 67.8 |
Redundancy | 4.6 | 3.5 |
CC(1/2) | 0.986 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25 % (w/v) PEG 3350 |