7TBQ
LOV2-DARPIN fusion : D7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 93.212, 93.212, 233.136 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.140 - 2.678 |
R-factor | 0.2418 |
Rwork | 0.239 |
R-free | 0.29490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7tbn |
RMSD bond length | 0.008 |
RMSD bond angle | 0.970 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.720 | 2.820 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.105 | |
Rmeas | 0.110 | |
Rpim | 0.034 | |
Number of reflections | 56150 | 2813 |
<I/σ(I)> | 12.8 | 1.5 |
Completeness [%] | 94.0 | 48.2 |
Redundancy | 10.1 | 10.2 |
CC(1/2) | 0.999 | 0.571 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM sodium chloride, 25% PEG3350, 100 mM Tris, pH 5.5 |