7TBP

X-ray structure of the HIV-1 myristoylated matrix protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-21
Detector	RAYONIX MX300-HS
Wavelength(s)	0.97954
Spacegroup name	H 3 2
Unit cell lengths	96.562, 96.562, 216.908
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.896 - 2.151
R-factor	0.1872
Rwork	0.184
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hiw
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.900	2.228
High resolution limit [Å]	2.150	2.150
Rmerge	0.047	0.573
Rmeas	0.051	0.633
Rpim	0.020	0.263
Number of reflections	21434	2087
<I/σ(I)>	21.66	2.21
Completeness [%]	99.6	98.86
Redundancy	6.1
CC(1/2)	0.999	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	40% PEG 400, 0.1 M Bis-Tris-propane buffer (pH 4.7)