7TBN
LOV2-DARPIN fusion : D11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.614, 84.367, 92.992 |
| Unit cell angles | 90.00, 115.73, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.760 |
| R-factor | 0.1975 |
| Rwork | 0.194 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tal |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.799 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.770 | 1.980 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.082 | |
| Rmeas | 0.088 | |
| Rpim | 0.034 | |
| Number of reflections | 60580 | 3030 |
| <I/σ(I)> | 12 | 1.6 |
| Completeness [%] | 92.7 | 60.9 |
| Redundancy | 12 | |
| CC(1/2) | 0.999 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 150 mM sodium formate, 18% PEG3350, 100 mM HEPES, pH 7.2 |
