7TA5
Crystal structure of cyanophycin synthetase 2 from Gloeothece citriformis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.5213 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 99.781, 99.781, 348.493 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 116.160 - 3.000 |
R-factor | 0.265 |
Rwork | 0.264 |
R-free | 0.28510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lgj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.660 |
Data reduction software | DIALS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2-4158_final: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 348.500 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.470 | 5.649 |
Number of reflections | 21675 | 3378 |
<I/σ(I)> | 18.3 | |
Completeness [%] | 100.0 | |
Redundancy | 105 | |
CC(1/2) | 0.998 | 0.451 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | tris-bicine pH8.5 200mM Na/K tartrate 60mM MgCl 10mM betaine 44% precip 2 4% 2,2,2-trifluoroethanol 10mM NiCl |